Scientists at Cambridge Biolabs are experts in Rt-qPCR and RNAi. We have spent years developing RNAi drug delivery systems for multiple companies. This translates to extensive experience with many qPCR systems that can help prove your drug or technology works. ViThera will use whichever platform option our clients prefer.
RNA isolation and QC
- Sample submission guidelines
- Phenol/chloroform extraction
- Direct-from-cells reverse transcription and amplification
RNA –based assays:
- RT-qPCR
- miRNA profiling
- Northern Blot
RNAi –specific
- Screening and selection of lead siRNA candidates
- Assessment of RNAi delivery technologies
- siRNA/shRNA technology evaluation
Data and analysis
- Samples run in triplicate
- Amplification curves and melt curves available where appropriate
- Raw Ct values provided
- ΔΔCt analysis provided with error bars
