Cambridge Biolabs brings Izon’s revolutionary qNano scanning ion occlusion sensing (SIOS) platform to the Kendall Square area.
CBL measures single particles for size and
size distribution analysis of multimodal
sample populations.
Particle Interaction Monitoring
We provide identification and analysis of
particle-particle, particle-biomolecule,
functionalization and aggregation interactions.
CBL’s relationship with Izon brings a
state-of-the-art solution for concentration
measurement in both biological and synthetic
particle samples.
qNano enables particle-by-particle collection &
assessment of polydisperse mixtures for highly
accurate determination of size distribution
within a sample.
The figure on the left shows a size distribution
histogram of a quad-modal mixture containing
four different polymer populations, measured
with the Izon qNano’s nanopore-based
detection (Inset shows particle diameter vs
translocation duration). Accurate size
distribution output is achieved with individual
particle detail.
Particle-particle interactions can be
carried out in situ in the upper fluid cell and the
interaction monitored in real-time through
changes in SIOS measurement parameters.
In the example to the left 10ng of avidin was
added to 184nm carboxylated polystyrene
particles in situ in the upper fluid cell. A clear
increase in the measured mean blockade
duration can be seen following introduction of
the avidin.
The relationship between an applied pressure
and the measured frequency of blockade
events is linear for a given particle set (see
figure at left).
Particle rates are proportional to concentration,
allowing the concentrations of two samples to
be compared through their measured blockade
frequency and gradient response over pressure.
Measurement of a standard at a single applied
pressure setting provides a reference against
which any other sample can be compared to
obtain an accurate absolute concentration
value.
